Journal: The Journal of antibiotics

Article Title: Activity of the streptogramin antibiotic etamycin against methicillin-resistant Staphylococcus aureus

doi: 10.1038/ja.2010.22

Figure Lengend Snippet: Antibacterial activity of Etamycin

Article Snippet: Gram negative strains used were Pseudomonas aeruginosa (ATCC 27853), Escherichia coli (ATCC 25922), Moraxella catarrhalis (ATCC 25238), and Haemophilus influenzae (Type b, ATCC 10211).

Techniques: Activity Assay

Journal:

Article Title: Rhamnolipid-Induced Removal of Lipopolysaccharide from Pseudomonas aeruginosa : Effect on Cell Surface Properties and Interaction with Hydrophobic Substrates

doi:

Figure Lengend Snippet: Effect of rhamnolipid on LPS released from cell suspensions of P. aeruginosa ATCC 27853. Cell suspensions were adjusted to an OD of 1.0 and were incubated with the respective rhamnolipid concentration for 8 h, and supernatant samples were assayed for LPS. Each point represents the average and standard deviation of three replicate samples.

Article Snippet: While release of LPS from ATCC 27853 could be described by a second-order polynomial curve ( r 2 = 0.977), release of LPS from ATCC 9027 proceeded in two phases: the first phase was characterized by a high, linear ( r 2 = 0.0995) rate of increase in LPS loss from 0 to 0.1 mm rhamnolipid and a subsequent lower linear ( r 2 = 0.990) rate of increase from 0.1 to 10 mM rhamnolipid. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG. 5 caption a7 Effect of rhamnolipid on LPS released from cell suspensions of P. aeruginosa ATCC 27853.

Techniques: Incubation, Concentration Assay, Standard Deviation

Journal:

Article Title: Rhamnolipid-Induced Removal of Lipopolysaccharide from Pseudomonas aeruginosa : Effect on Cell Surface Properties and Interaction with Hydrophobic Substrates

doi:

Figure Lengend Snippet: Effect of rhamnolipid on LPS released from cell suspensions of P. aeruginosa ATCC 9027. Cell suspensions were adjusted to an OD of 1.0 and were incubated with the respective concentration of rhamnolipid for 8 h, and supernatant samples were assayed for LPS. Each point represents the average and standard deviation of three replicate samples.

Article Snippet: While release of LPS from ATCC 27853 could be described by a second-order polynomial curve ( r 2 = 0.977), release of LPS from ATCC 9027 proceeded in two phases: the first phase was characterized by a high, linear ( r 2 = 0.0995) rate of increase in LPS loss from 0 to 0.1 mm rhamnolipid and a subsequent lower linear ( r 2 = 0.990) rate of increase from 0.1 to 10 mM rhamnolipid. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG. 5 caption a7 Effect of rhamnolipid on LPS released from cell suspensions of P. aeruginosa ATCC 27853.

Techniques: Incubation, Concentration Assay, Standard Deviation

Journal:

Article Title: Rhamnolipid-Induced Removal of Lipopolysaccharide from Pseudomonas aeruginosa : Effect on Cell Surface Properties and Interaction with Hydrophobic Substrates

doi:

Figure Lengend Snippet: A silver-stained SDS-PAGE of concentrated (10×) supernatants of suspensions of P. aeruginosa ATCC 27853. Lane 1, buffer; lane 2, 300 μg of rhamnolipid; lane 3, 5 μg of bovine serum albumin; lane 4, supernatant of P. aeruginosa ATCC 27853 treated only with MSM for 8 h; lane 5, supernatant of P. aeruginosa ATCC 27853 treated with 6 mM rhamnolipid for 8 h; lane 6, supernatant of P. aeruginosa ATCC 27853 treated only with MSM for 24 h; lane 7, supernatant of P. aeruginosa ATCC 27853 treated with 6 mM rhamnolipid for 24 h; lane 8, 5 μg of P. aeruginosa serotype 10 LPS; lane 9, 50 μg of P. aeruginosa serotype 10 LPS; lane 10, 1 μg of P. aeruginosa serotype 10 LPS.

Article Snippet: While release of LPS from ATCC 27853 could be described by a second-order polynomial curve ( r 2 = 0.977), release of LPS from ATCC 9027 proceeded in two phases: the first phase was characterized by a high, linear ( r 2 = 0.0995) rate of increase in LPS loss from 0 to 0.1 mm rhamnolipid and a subsequent lower linear ( r 2 = 0.990) rate of increase from 0.1 to 10 mM rhamnolipid. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG. 5 caption a7 Effect of rhamnolipid on LPS released from cell suspensions of P. aeruginosa ATCC 27853.

Techniques: Staining, SDS Page